- Define novel proteins that interact with IGF-I receptor or insulin receptor by two-hybrid assay, or co-immunoprecipitation proteomics analysis, and elucidate physiological roles of these proteins
- Insulin receptor or insulin-like growth factor I receptor-associated proteins (InsRAPs and IGFRAPs)
- 14-3-3, SOCS proteins, Grb10, PI 3-kinase
- Define novel proteins that interact with IRS family proteins by two-hybrid assay and co-immunoprecipitation proteomics analysis
- Signal-modulating proteins; 14-3-3
- Endosome sorting proteins; AP-1 and AP-2 components, importin
- Transcription regulator proteins; TYSLP, BMI
- Phosphorylation modulators; 53BP2, DGK, IRSAL
- Ubiquitination and deubiquitination enzymes; Nedd4, Mdm2, and UPSs
- Phosphorylation and dephosphorylation enzymes; JNK, and S6K
- Regulators of RNA metabolism; PABP
- Proteins containing other activities: Tip-60, Sirt1, and Pin1
- Chaperones; gC1qR, HSP90
- Define the mechanisms of regulation of localization of IRS family proteins and their function
- Cytosolic localization of IRS-1 and IRS-2
- Endosomal localization of IRS-1 and IRS-2
- Mitochondrial localization of IRS-1 and IRS-2
- Nuclear localization of IRS-3
- Characterize intramolecular modification of IRS family proteins under various conditions.
- Screening of small molecules for capacity to modulate binding between IRS and IRS-associated proteins, IGF-I receptor and associated proteins, and insulin receptor and associated proteins
- Small compounds in chemical library in Riken
- Indian medicine, and Kampo medicine
- Food factors
|