Project

Project 3 (2)

   Recently, we initiated studies to identify proteins forming a complex with IRSs by peptide mass fingerprinting (PMF) analysis of co-immunoprecipitates using anti-IRSs antibodies which we prepared. According to this method, we can show that IRSs make a complex with chaperon molecules, ubiquitin ligase, and proteins that play important roles in protein synthesis, etc (Figs. 9 and 10).

   In addition, we are working on the proteins that are associated with IGF-I receptor and/or insulin receptor and function as a modulator of IGF/insulin signals. Analysis of intramolecular modifications of IRS family proteins is also in progress in our laboratory (Fig. 11).

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Figure 9. Methods to identify IRS-associated proteins and/or proteins forming IRSome

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Figure 10. Physiological roles of IRS-associated proteins that have been identified

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Figure 11. Intramolecular modification of IRS and binding with IRS-associated proteins may modulate IGF/insulin signaling

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Project 3 (2)

Recently, we initiated studies to identify proteins forming a complex with IRSs by peptide mass fingerprinting (PMF) analysis of co-immunoprecipitates using anti-IRSs antibodies which we prepared. According to this method, we can show that IRSs make a complex with chaperon molecules, ubiquitin ligase, and proteins that play important roles in protein synthesis, etc (Figs. 9 and 10). In addition, we are working on the proteins that are associated with IGF-I receptor and/or insulin receptor and function as a modulator of IGF/insulin signals. Analysis of intramolecular modifications of IRS family proteins is also in progress in our laboratory (Fig. 11).

open this figure in another window	Figure 9. Methods to identify IRS-associated proteins and/or proteins forming IRSome

open this figure in another window	Figure 10. Physiological roles of IRS-associated proteins that have been identified

open this figure in another window	Figure 11. Intramolecular modification of IRS and binding with IRS-associated proteins may modulate IGF/insulin signaling

back next